Alanine - Aminopeptidase (Microsomale Aap), γ-Glutamyl Transferase (γ-Gt), β2- Microglobulin (β2-M) Enzymuria As Screening Test In Patients With Degenerative Spondyloarthropaty

Abstract

Objectives: To compare diagnostic values and laboratory variables of alanine-aminopeptidase (microsomale AAP), γ-glutamyl transferase (γ-GT), β2-microglobuline (β2-M), in early diagnosis in previously untreated degenerative spondyloarthropaty (DSA). To determine the effect of untreated DSA on tubular function, as well as the diagnostic value of the enzymes originating from proximal renal tubules. Material and Methods: From the standard methods of the International Federation for Clinical Chemistry (IFCC) we used the kinetic method for determination of alanine - aminopeptidase (microsomal AAP), γ-glutamyl transferase (γ-GT) and MEIA (Microparticles Enzyme Immunoassay(Abbot Axsym system) for determination of β2-microglobuline in urine. We examined samples (serum and urine) from 70 participants (35 DSA untreated, 35 healthy control group). Results: From 35 examined patients with DSA, 12 pts showed presence of AAP enzymuria (test sensitivity was 34.28%), 8 pts showed presence of γ-GT (test sensitivity was 22.85%), while the presence of β2-microglobuline in urine was low (test sensitivity 0%). Conclusion: AAP has better sensitivity than γ-GT and β2-microglobuline in the detection of asymptomatic renal endothelial changes in untreated DSA.